Rapid detection of bacterial pathogens is critical toward judicious management of infectious diseases.
Herein, we demonstrate an in situ electrokinetic stringency control approach for a self-assembled monolayer-based electrochemical biosensor toward urinary tract infection diagnosis. The in situ electrokinetic stringency control technique generates Joule heating induced temperature rise and electrothermal fluid motion directly on the sensor to improve its performance for detecting bacterial 16S rRNA, a phylogenetic biomarker. The dependence of the hybridization efficiency reveals that in situ electrokinetic stringency control is capable of discriminating single-base mismatches. With electrokinetic stringency control, the background noise due to the matrix effects of clinical urine samples can be reduced by 60%. The applicability of the system is demonstrated by multiplex detection of three uropathogenic clinical isolates with similar 16S rRNA sequences. The results demonstrate that electrokinetic stringency control can significantly improve the signal-to-noise ratio of the biosensor for multiplex urinary tract infection diagnosis.
Written by:
Liu T, Sin ML, Pyne JD, Gau V, Liao JC, Wong PK. Are you the author?
Department of Aerospace and Mechanical Engineering, University of Arizona, Tucson, AZ, USA.
Reference: Nanomedicine. 2013 Jul 24. pii: S1549-9634(13)00346-8.
doi: 10.1016/j.nano.2013.07.006
PubMed Abstract
PMID: 23891989
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