AUA 2018: Transdifferentiation Form White Adipocytes to Beige Cells Prevents Kidney Stone Formation

San Francisco, CA USA (UroToday.com) Dr. Teruaki Sugino and his colleagues report the effects transdifferentiating white adipocytes to beige cells using a treatment of 1.0mg/kg β3-adrenergic receptor agonist CL316243 on the formation of kidney stones in C57BL/6 mice. The team utilized a control group that received saline injections (n=20) and the treatment group (n=20). Treatments were done for 12 days and from days 6-12, 80mg/kg of glyoxylate were injected intra-abdominally to induce renal crystal deposits.

Data reported included histological observations, polarized light microscopy for quantification of renal crystal deposits, quantitative reverse transcriptase polymerase chain reaction of uncoupling protein 1 (UCP1), adipocytokine, stone-related genes (SOD1, EMR1, Pgc1, and Spp1).

Data was collected at days 0, 6, and 12. Expression of UCP1 and adiponectin in the β3-treated group increased by 8.1 and 4.2-fold, respectively, compared with those from the control group (p<0.01, p=0.01). Expression if IL-6 and osteopontin decreased by 0.3-fold and 0.1-fold (p=0.03, p=0.02), while the levels of SOD1 increased by 2.3-fold and EMR1 decreased by 0.4-fold (p=0.04). Notably, the formation of renal crystal deposits decreased to 17.0% (p=0.03) in the β3-experimental group. The data showed no significant effect of the treatments on body weight and urine biochemistry.

The team concludes β3-adrenergic receptor agonist treatment decreases pro-inflammatory adipocytokines and increases antioxidants, indicating the transdifferentiating of white adipocytes to beige cells could decrease the formation of renal stones.

Their future work will be to observe more specific details and mechanisms of white adipocytes versus beige cells and their methods of influencing oxidative stress, inflammation, and renal stone formation.

Presenter(s): Teruaki Sugino

Co-Authors: Maarten Hulshof, André Vis, Koos Zwinderman, Jos Twisk, Karl Delaere, Jeroen van Moorselaar, Paul Verhagen, George van Andel

Written by: Victoria Lee, MD, Clinical Fellow, Department of Urology, University of California-Irvine at the 2018 AUA Annual Meeting - May 18 - 21, 2018 – San Francisco, CA USA

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