AUA 2024: Phase I Study of Intravesical Fc-Optimized Anti-CD40 Agonist Antibody 2141-V11 for Non-Muscle Invasive Bladder Cancer (NMIBC) Unresponsive to Bacillus Calmette-Guerin (BCG)

(UroToday.com) The 2024 American Urological Association (AUA) Annual Meeting held in San Antonio, TX was host to a non-invasive bladder cancer moderated poster session. Dr. Bernard Bochner presented the results of a phase 1 study of intravesical Fc-optimized anti-CD40 agonist antibody 2141-V11 for non-muscle invasive bladder cancer (NMIBC) unresponsive to Bacillus Calmette-Guerin (BCG).

2141-V11 is a novel anti-CD40 antibody developed with enhanced binding to FcγRIIB resulting in effective tumor-specific T-cell responses in vivo. In preclinical bladder cancer models, including BCG-unresponsive disease, intravesical 2141-V11 results in durable anti-tumor immunity without systemic toxicity. Based on these findings, Dr. Bochner and colleagues initiated a first in human phase I/II study of intravesical 2141-V11 for the treatment of BCG-unresponsive NMIBC.

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This is an investigator-initiated phase I, open-label, dose-escalation study to evaluate the safety and tolerability of intravesical 2141-V11 in patients with BCG-unresponsive NMIBC who are ineligible for or decline radical cystectomy (NCT05126472; N=25). Following complete transurethral resection, intravesical 2141-V11 was administered once weekly for three doses with re-treatment eligibility at weeks 13 and 25, depending on disease state. Dose escalation follows a modified continual reassessment method (MCRM) design. Primary endpoints were safety and dose tolerability to determine the maximal tolerated dose (MTD) and/or recommended phase II dose. Secondary endpoints include pharmacokinetics and preliminary evaluation of clinical activity. Exploratory aims include investigation of biological markers of drug activity in tissue and urine. 

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This analysis included 21 patients to date. Patients included had carcinoma in situ (CIS) with or without Ta/T1 (N=15) or Ta/T1 without CIS (N=6). Ten patients had a prior history of T1 disease. Patients received a mean of 12.7 doses of prior BCG.

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Intravesical 2141-V11 was well tolerated (no grade ≥3 events) with no dose-limiting toxicities up to the highest tested dose of 70 mg. MTD was not reached.

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With regards to efficacy outcomes, at the 3-month timepoint (n=20 evaluable patients), 17/20 did not develop HG T1 disease six (30%) demonstrated a complete response. At the 6-month timepoint (n=16 evaluable patients), 7/16 (44%) had achieved a complete response. A total of 9.20 (45%) demonstrated a complete response at any time point.

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Post-treatment urine studies reveal increased neutrophils/neutrophil-related cytokines, particularly in responders. Single-cell spatial phenotyping is ongoing.

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Dr. Bochner concluded that intravesical 2141-V11 is well-tolerated with no dose-limiting toxicities observed up to 70 mg. MTD was not reached. Despite nearly half the population having a history of T1 disease prior to enrollment, 44% demonstrated a complete response at 6 months with a complete response rate of 45% at any time point. Correlative studies are ongoing to further investigate on-target activity in post-treatment specimens.

Presented by: Bernard Bochner, MD, FACS, Professor, Sir Murray Brennan Endowed Chair in Surgery, Memorial Sloan Kettering Cancer Center, New York, NY

Written by: Rashid Sayyid, MD, MSc – Society of Urologic Oncology (SUO) Clinical Fellow at The University of Toronto, @rksayyid on Twitter during the 2024 American Urological Association (AUA) Annual Meeting, San Antonio, TX, Fri, May 3 – Mon, May 6, 2024.