Direct evidence for the relationship between a large prostate (≥80 ml) and androgen receptor/PSA signal remains lacking in benign prostatic hyperplasia (BPH). Our aim is to identify whether the cause of a large prostate is related to progesterone receptor (PGR) androgen receptor (AR), oestrogen receptor α, β (ERα,β) and prostate-specific antigen (PSA).
Surgical specimens of BPH in plasmakinetic resection of the prostate (PKRP) with three groups of different prostate-sizes with mean volumes of 25.97 ml, 63.80 ml, and 122.37 ml were collected for immunohistochemical analysis of the tissue microarray with PGR, AR, PSA and ERs. Rats were castrated and treated with testosterone replacement to explore androgen and PGR, AR and ERs expression levels in the prostate. Quantitative real-time reverse transcription polymerase chain reaction (Rt-PCR) for mRNA detection of above genes was conducted.
Immunoblotting, Rt-PCR and immunohistochemistry assays showed that PGR, PSA, AR, ERα expression levels were positively correlated with prostate size and that ERβ expression levels were negatively correlated with prostate volume. Animal experiments have shown that prostate volume is decreased in castrated rats with decreased PGR, AR, ERα and increased ERβ expression levels.
PGR, AR, ERs signals can be regarded as important factors for large-sized prostates in BPH patients (≥100 ml).
The aging male : the official journal of the International Society for the Study of the Aging Male. 2024 Aug 14 [Epub]
Haohan Wang, Chengcheng Liu, Ziqiang Dong, Xiaobo Chen, Ping Zhang
Department of Urology, Yichang Central People's Hospital, The First Clinical Medical College, Three Gorges University, Yichang, Hubei, PR China., Department of Obstetrics and Gynaecology, Yichang Central People's Hospital, The First Clinical Medical College, Three Gorges University, Yichang, Hubei, PR China.