The aim of this study was to evaluate conventional semen parameters (density, morphology and progressive motility) and the flow-cytometric parameters DNA fragmentation, mitochondrial membrane potential, phosphatidylserine externalization and chromatin compactness in patients with varicocele before and after varicocelectomy.
Thirty men (26.5±3.2 years, range 20-32 years) with oligoasthenoteratozoospermia and grade 3 left varicocele were selected (without other causes of male infertility). Each of them underwent sperm analysis and flow cytometry evaluation before and 4 months after subinguinal microsurgical varicocelectomy (SMV).
After varicocelectomy, men had significantly higher sperm density, progressive motility and normal forms compared to baseline. They also had a significantly lower percentage of spermatozoa with low mitochondrial membrane potential. After SMV, they showed a significantly lower percentage of spermatozoa with PS externalization, an early sign of apoptosis. Significantly decreased percentages of spermatozoa with abnormal chromatin compactness and spermatozoa with DNA fragmentation were found after SMV compared to baseline.
Subinguinal microsurgical varicocelectomy improves sperm function in oligoasthenoteratozoospermia secondary to grade 3 left varicocele. Improvements are seen in conventional parameters and biofunctional parameters not routinely evaluated.
Written by:
La Vignera S, Condorelli R, Vicari E, D'Agata R, Calogero AE. Are you the author?
Reference: J Androl. 2011 Jun 2. Epub ahead of print.
doi: 10.2164/jandrol.111.013433
PubMed Abstract
PMID: 21636732
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