Section of Endocrinology, Andrology and Internal Medicine and Master in Andrological, Human Reproduction and Biotechnology Sciences, Department of Biomedical Sciences, University of Catania, Catania, Italy.
Male accessory gland infections (MAGI) represent a major cause of male infertility mainly through the secretory dysfunction of the prostate, seminal vesicles and epididymis. This study was undertaken to evaluate conventional and non-conventional sperm parameters in these patients, therefore 150 patients with MAGI were selected. Each of them underwent to two sperm analyses and evaluation of DNA fragmentation mitochondrial membrane potential (MMP), phosphatidylserine (PS) externalization, chromatin compactness, by flow cytometry. Results showed that patients with MAGI had a lower sperm progressive motility (11.4±5.0vs34.0±7.0%), and percentage of normal forms (9.0±3.7vs33.0±13.0%) compared to controls, instead, these patients showed higher number of seminal white blood cells (2.2±1.0vs0.4±0.6 10637 /ml). Patients with MAGI showed a higher number of spermatozoa with DNA fragmentation compared to controls 39 (8.2±3.0vs1.0±1.0%). In addition they have also a higher percentage of spermatozoa with low MMP (28.0±4.0vs2.0±2.0%). Patients with MAGI, showed a higher percentage of spermatozoa with PS externalization (8.0±4.0vs3.0±3.0%), an early sign of apoptosis, and lower percentage of viable spermatozoa (64.5±12.0vs88.0±10.0%). An increased percentage of spermatozoa with abnormal chromatin compactness (18.0±4.0vs5.0±3.0%) was found in patients with MAGI. In conclusion patients with MAGI show alterations of conventional and biofunctional sperm parameters compared to controls. These results suggest to consider the flow cytometry evaluation among the diagnostic tools for male infertility.
Written by:
La Vignera S, Condorelli R, D'Agata R, Vicari E, Calogero AE. Are you the author?
Reference: J Endocrinol Invest. 2011 Sep 23. Epub ahead of print.
PubMed Abstract
PMID: 21946047
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