To investigate the diagnostic values of urine cytokines in interstitial cystitis/bladder pain syndrome (IC/BPS) patients and to identify their correlations with clinical characteristics.
Urine samples were collected from 127 IC/BPS (ESSIC types 1 and 2) patients and 28 controls. Commercially available multiplex immunoassays (MILLIPLEX® map kits) were used to analyze 31 targeted cytokines. Cytokine levels between IC/BPS patients and controls were analyzed using ANOVA. Receiver operating characteristics (ROC) curves of each cytokine to distinguish IC/BPS from controls were generated for calculation of the area under the curve (AUC).
IC/BPS Patients had urine cytokine profiles that differed from those of controls. Between ESSIC types 1 and 2 IC/BPS patients, urine cytokine profiles were also different. Among cytokines with high diagnostic values (i.e., AUC > 0.7) with respect to distinguish ESSIC type 2 IC/BPS patients from controls, RANTES, MIP-1β, and IL-8 were of higher sensitivity, whereas MCP-1, CXCL10, and eotaxin-1 were of higher specificity. Multivariate logistic regression models for controlling for age, gender, body mass index, and diabetes mellitus, the urine cytokines with high diagnostic values (MCP-1, RANTES, CXCL10, IL-12p40, IL-7, and eotaxin-1) remained statistically significant in differentiating IC/BPS and controls. MCP-1, CXCL10, eotaxin-1, and RANTES were positively correlated with glomerulation grade and negatively correlated with maximal bladder capacity.
IC/BPS patients had urine cytokine profiles that clearly differed from those of controls. Urine cytokines might be useful as biomarkers for diagnosing IC/BPS and mapping its clinical characteristics.
American journal of physiology. Renal physiology. 2020 Apr 13 [Epub ahead of print]
Yuan-Hong Jiang, Jia-Fong Jhang, Yung-Hsiang Hsu, Han-Chen Ho, Ya-Hui Wu, Hann-Chorng Kuo
Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Taiwan., Department of Pathology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Taiwan., Department of Anatomy, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Taiwan.