PURPOSE: To evaluate prostaglandin E2 (PGE2) and E-series prostaglandin (EP) receptor in patients with interstitial cystitis (IC).
MATERIALS AND METHODS: Twenty female patients with IC (11 with and 9 without Hunner lesions), 9 female controls with other urological diseases who needed cystoscopic procedure and 10 normal volunteers were enrolled. O'Leary-Sant Score (OSS) for symptoms and problems and voluntary urine for PGE2 analysis were obtained from all subjects. Under anesthesia, the bladder was distended by saline in a stepwise fashion (from 100ml to maximum capacity) in patients with IC, and the infused saline was retrieved each time for PGE2 analysis. We also measured PGE2 and expression of EP receptors mRNA in bladder biopsy tissue in patients with IC.
RESULTS: Symptoms and problems index in IC patients with Hunner lesions was significantly higher than IC patients without Hunner lesions. Urinary PGE2 in IC patients with Hunner lesions was significantly higher than IC patients without Hunner lesions, controls and normal volunteers. PGE2 level in retrieved saline in IC patients with Hunner lesions increased depending on infusion volume, but not in IC patients without Hunner lesions. PGE2 content in bladder biopsy tissue was significantly higher in IC patients with Hunner lesions than controls. In IC patients with Hunner lesions, expression of EP1 and EP2 mRNA was significantly higher than controls.
CONCLUSIONS: The present study showed an increase in PGE2 production and mRNA expression of EP1 and EP2 receptors in the bladder in IC patients with Hunner lesions. Further studies are warranted to explore its pathophysiological and therapeutic implication.
Written by:
Wada N, Ameda K, Furuno T, Okada H, Date I, Kakizaki H. Are you the author?
Department of Renal and Urologic Surgery, Asahikawa Medical University, Asahikawa, Japan; Department of Urology, Hokkaido Memorial Hospital of Urology, Sapporo, Japan; Ono Pharmaceutical Co., Ltd, Minase Research Institute, Osaka, Japan.
Reference: J Urol. 2015 Jan 13. pii: S0022-5347(15)00031-2.
doi: 10.1016/j.juro.2015.01.010
PubMed Abstract
PMID: 25595860