To investigate the efficacy of muscle-derived stem cells (MDSCs) combined with nerve growth factor (NGF) in the treatment of stress urinary incontinence (SUI) METHODS: MDSCs were isolated and extracted from 90 SD rats, and the stem cell characteristics of the cells were identified using flow cytometry. NGF overexpression (oe-NGF) plasmid was coated with adenovirus and qRT-PCR was applied to verify adenovirus transfection efficiency. The rat models of SUI were constructed and randomly divided into five groups: control group, phosphate buffer (PBS) group, MDSCs + oe-NGF group, MDSCs + vector group, and MDSCs group. After 8 weeks of feeding, the leakage point pressure (LPP) rats and Masson staining of rat urethral sections were detected. The expression of NGF and vascular endothelial growth factor (VEGF) was detected by western blot and IHC staining RESULTS: Compared with the control group, the LPP and the ratio of muscle fibers/collagen fibers were significantly increased in the MDSCs treated groups, with the highest increase in the MDSCs + oe-NGF group. Western blot and IHC results showed that the expression of NGF and VEGF in the urethral tissues in the MDSCs treated groups were significantly up-regulated comparing with the control group, with the highest increase in the MDSCs + oe-NGF group CONCLUSION: MDSCs alone can relieve SUI, while MDSCs combined with NGF is more effective, which may be related to the up-regulating of VEGF.
Urology. 2022 Apr 28 [Epub ahead of print]
Jianxiong Fang, Tianming Peng, Jiumin Liu, Haosheng Liu, Tianqi Liu, Zhenhui Zhang, Chao Zhao, Yong Li, Qianqian Wang, Hanzhong Chen, Teng Li, Shang Huang, Xiaoyong Pu
Jiangmen Central Hospital, Jiangmen, P.R.China; Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, P.R.China., Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, P.R.China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, P.R.China., Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, P.R.China., Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, P.R.China; Shantou University Medical College, Shantou, P.R.China., Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, P.R.China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, P.R.China. Electronic address: .