Detecting pre-clinical bladder cancer (BC) using urinary biomarkers may provide a valuable opportunity for screening and management. Telomerase reverse transcriptase (TERT) promoter mutations detectable in urine have emerged as promising BC biomarkers.
We performed a nested case-control study within the population-based prospective Golestan Cohort Study (50,045 participants, followed up to 14 years) and assessed TERT promoter mutations in baseline urine samples from 38 asymptomatic individuals who subsequently developed primary BC and 152 matched controls using a Next-Generation Sequencing-based single-plex assay (UroMuTERT) and droplet digital PCR assays.
Results were obtained for 30 cases and 101 controls. TERT promoter mutations were detected in 14 pre-clinical cases (sensitivity 46·67%) and none of the controls (specificity 100·00%). At an estimated BC cumulative incidence of 0·09% in the cohort, the positive and negative predictive values were 100·00% and 99·95% respectively. The mutant allelic fractions decreased with the time interval from urine collection until BC diagnosis (p = 0·033) but the mutations were detectable up to 10 years prior to clinical diagnosis.
Our results provide the first evidence from a population-based prospective cohort study of the potential of urinary TERT promoter mutations as promising non-invasive biomarkers for early detection of BC. Further studies should validate this finding and assess their clinical utility in other longitudinal cohorts.
French Cancer League, World Cancer Research Fund International, Cancer Research UK, Tehran University of Medical Sciences, the International Agency for Research on Cancer, and the U.S. National Cancer Institute.
EBioMedicine. 2020 Feb 12 [Epub ahead of print]
Md Ismail Hosen, Mahdi Sheikh, Maria Zvereva, Ghislaine Scelo, Nathalie Forey, Geoffroy Durand, Catherine Voegele, Hossein Poustchi, Masoud Khoshnia, Gholamreza Roshandel, Masoud Sotoudeh, Arash Nikmanesh, Arash Etemadi, Patrice Hodonou Avogbe, Priscilia Chopard, Tiffany Myriam Delhomme, Matthieu Foll, Arnaud Manel, Emmanuel Vian, Elisabete Weiderpass, Farin Kamangar, Paolo Boffetta, Paul D Pharaoh, Sanford M Dawsey, Christian C Abnet, Paul Brennan, James McKay, Reza Malekzadeh, Florence Le Calvez-Kelm
International Agency for Research on Cancer (IARC), Lyon, France; Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh., International Agency for Research on Cancer (IARC), Lyon, France; Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran., International Agency for Research on Cancer (IARC), Lyon, France; Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia., International Agency for Research on Cancer (IARC), Lyon, France., Digestive Disease Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran., Digestive Disease Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran., Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran., Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran., Digestive Disease Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Metabolic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA., Protestant Clinic of Lyon, Urology department, Lyon, France., Department of Biology, School of Computer, Mathematical, and Natural Sciences, Morgan State University, Baltimore, MD, USA., Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Department of Medical and Surgical Sciences, University of Bologna, Italy., Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom., Metabolic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA., Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Digestive Disease Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: ., International Agency for Research on Cancer (IARC), Lyon, France. Electronic address: .
PubMed http://www.ncbi.nlm.nih.gov/pubmed/32081602