A novel therapeutics agent: antioxidant effects of hydroxylfasudil on rat kidney and liver tissues in a protamine sulphate-induced cystitis rat model; preliminary results

Cystitis is defined as an inflammation of the bladder caused by a bacterial infection, and it can be dangerous and painful when it spreads through the internal organs. In this study, antioxidant effects of hydroxylfasudil (HF) at the enzymatic and molecular level on kidney and liver tissues in cystitis rat model, which is caused by inflammation of the rat bladder with a protamine sulphate (PS), was examined. Quantitative changes of reduced glutathione (GSH) and lipid peroxidation (LPO) levels, which are a marker for oxidative stress, were determined in rat kidney and liver tissues for each groups. And then molecular and biochemical impact of HF treatment on antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) in cystitis model were studied. The results suggest that HF could be beneficial to the renal and hepatic antioxidant system. Thus, HF might be used as a novel therapeutics agent to eliminate interstitial cystitis.

Artificial cells, nanomedicine, and biotechnology. 2018 Mar 09 [Epub ahead of print]

Aliseydi Bozkurt, Harun Budak, Huseyin Serkan Erol, Serpil Can, Tolga Mercantepe, Yigit Akin, Isa Ozbey, Murat Cankaya, Mesut Bunyamin Halici, T Abdulkadir Coban

a Department of Urology , Erzincan University , Erzincan , Turkey., b Department of Molecular Biology and Genetics , Ataturk University , Erzurum , Turkey., c Department of Biochemistry , Ataturk University , Erzurum , Turkey., d Department of Physiology , Kafkas University , Kars , Turkey., e Department of Histology-Embryology , Recep Tayyip Erdoğan University , Rize , Turkey., f Department of Urology , Izmir Katip Celebi University , Izmir , Turkey., g Department of Urology , Ataturk University , Erzurum , Turkey., h Department of Biology , Erzincan University , Erzincan , Turkey., i Department of Biochemistry , Erzincan University , Erzincan , Turkey.