Department of Nuclear Medicine, University Hospital Gasthuisberg Leuven, Leuven, Belgium.
The aim of this study was to evaluate the impact of androgen ablation therapy in different prostate cancer (PCa) cell lines-reflecting different stages of the disease-on (18)F-fluorodeoxyglucose (FDG), (11)C-choline and (11)C-acetate uptake.
Uptake experiments were performed in androgen-sensitive (LNCaP, PC346C) and independent cell lines (22Rv1, PC346DCC, PC-3) as well as in a benign prostatic hyperplasia (BPH-1) cell line. Tracer uptake was assessed under androgen ablation. Results of the cancer cell lines were normalized to those of BPH-1. To evaluate the effect of androgen on the uptake of (18)F-FDG, (11)C-choline and (11)C-acetate in PCa cell lines, 10(-8)M R1881, 10(-10)M R1881, the combination of 10(-10)M R1881 plus 10(-6)M Casodex or 10(-6)M Casodex alone were added in parallel cell cultures 1 day before uptake experiments. Uptake in androgen-supplemented cell cultures was compared to the uptake under androgen deprivation. Uptake was corrected for cell number using protein content.
Compared to BPH-1, a higher (18)F-FDG uptake was observed only in PC346C cells, whereas a higher (11)C-choline and markedly increased (11)C-acetate uptake was seen in all cancer cell lines. Androgens significantly modulated the uptake of (18)F-FDG in LNCaP, PC346C and 22Rv1 cells, and of (11)C-choline in the PC346C and 22Rv1 cell line. No androgenic effect on (11)C-choline and (18)F-FDG uptake was observed in PC-3 and PC346DCC cells. (11)C-Acetate uptake was independent of androgen status in all PCa cell lines studied.
(18)F-FDG uptake in PCa cell lines showed the highest variability and strongest androgen effect, suggesting its poor potential for metabolic imaging of advanced PCa. In contrast to (18)F-FDG and (11)C-choline, (11)C-acetate uptake was unaffected by androgens and thus (11)C-acetate seems best for monitoring PCa progression.
Written by:
Emonds KM, Swinnen JV, van Weerden WM, Vanderhoydonc F, Nuyts J, Mortelmans L, Mottaghy FM. Are you the author?
Reference: Eur J Nucl Med Mol Imaging. 2011 Jul 6. Epub ahead of print.
doi: 10.1007/s00259-011-1861-6
PubMed Abstract
PMID: 21732108
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