Urology, San Francisco Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA.
University of California at San Francisco, San Francisco, CA.
The purpose of this study was to identify new tumor suppressor microRNAs (miRs) in bladder cancer, carry out functional analysis of their suppressive role and identify their specific target genes. To explore tumor suppressor miRs in bladder cancer, miR microarray was performed using SV-HUC-1, T24, J82 and TCCSUP cells. Expression of miR-493 in bladder cancer (T24, J82 and TCCSUP) cells was down-regulated compared to normal SV-HUC-1 cells. Also the expression of miR-493 was significantly lower in bladder cancer tissues than in their corresponding non-cancerous tissues. Transfection of miR-493 into T24 or J82 cells decreased their cell growth and migration abilities. On the basis of this result, to identify potential miR-493 target genes, we used target scan algorithms to identify target oncogenes related to invasion and migration. MiR-493 decreased 3'UTR luciferase activity and protein expression of FZD4 and RhoC. MiR-493 also decreased binding of RhoC and Rock-1. MiR-493 is a new tumor suppressor microRNA in bladder cancer and inhibits cell motility through down-regulation of RhoC and FZD4.
Written by:
Ueno K, Hirata H, Majid S, Yamamura S, Shahryari V, Tabatabai ZL, Hinoda Y, Dahiya R. Are you the author?
Reference: Mol Cancer Ther. 2011 Nov 4. Epub ahead of print.
doi: 10.1158/1535-7163.MCT-11-0592
PubMed Abstract
PMID: 22057916
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