Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site.
Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.
Written by:
Zhao ZY, San M, Duprey JL, Arrand JR, Vyle JS, Tucker JH. Are you the author?
School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Reference: Bioorg Med Chem Lett. 2012 Jan 1;22(1):129-32. Epub 2011 Nov 20.
PubMed Abstract
PMID: 22169264
UroToday.com Investigational Urology Section
