As a key glycolytic enzyme, enolase 1 (ENO1) is critical for cellular energy metabolism. Recent studies have revealed its important role in growth and metastasis of lung, head and neck, and breast cancer. However, the regulatory mechanisms of ENO1 expression and secretion remain unclear. We observed that conditioned medium from estradiol-stimulated prostate stromal cells significantly promoted the migration of prostate cancer (PCa) cells. Two-dimensional protein electrophoresis, mass spectrometry, and immunodepletion assays identified one of the major active factors in the conditioned medium as α-type enolase (α-enolase, or ENO1). Moreover, in prostate stromal cells, estradiol not only enhanced the stability of ENO1 at the protein level in an estrogen receptor-α-dependent manner but also promoted its secretion to the extracellular matrix. Furthermore, recombinant ENO1 bound to the surface of PCa cells and promoted cell migration via their plasminogen receptor activity in a paracrine manner. Immunohistochemistry suggested that stromal ENO1 levels increased in PCa compared with those in normal tissue.
Written by:
Yu L, Shi J, Cheng S, Zhu Y, Zhao X, Yang K, Du X, Klocker H, Yang X, Zhang J Are you the author?
Departments of Biochemistry (L.Y.) and Pathology (X.Z.), Basic Medical College, Tianjin Medical University, Tianjin 300070, China; Department of Biochemistry and Molecular Biology (L.Y., J.S., S.C., X.D., J.Z.), College of Life Sciences, and State Key Laboratory of Medicinal Chemical Biology (J.Z.), Nankai University, Tianjin 300071, China; Tianjin State Key Laboratory of Modern Chinese Medicine (Y.Z.), Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; Institute of Urology (K.Y.), The Second Hospital of Tianjin Medical Universtiy, Tianjin 300211, China; Department of Urology (H.K.), Medical University of Innsbruck, A-6020 Innsbruck, Austria; and Medical Scientific Research Center of Guangxi Medical University (X.Y.), Nanning 530021, China.
Reference: Mol Endocrinol. 2012 Jun 25. [Epub ahead of print]
doi: 10.1210/me.2012-1006
PubMed Abstract
PMID: 22734040
