OBJECTIVE:To investigate the expression of Jade-1 and β-catenin and their effect in the development of renal cell carcinoma (RCC).
METHODS:The expression of Jade-1 and β-catenin in 11 normal kidney tissue specimens (normal group) and 60 RCC specimens (RCC group) was determined using real-time polymerase chain reaction and immunohistochemistry. Also, their effect on early relapses of RCC was analyzed after 5 years of follow-up.
RESULTS:The expression of Jade-1 protein in the RCC group was significantly lower than that in the normal group (0.1655 vs 0.7438, P < .05), and the expression of β-catenin protein was significantly greater than that in the normal group (0.2756 vs 0.0855, P < .05). The expression of Jade-1 mRNA in the RCC group was 0.202 times that in the normal group, which were lower (P < .05). The expression of β-catenin mRNA was 1.014 times that in the normal group (P > .05). The expression of Jade-1 protein and β-catenin protein in poorly differentiated RCC specimens was significantly lower and higher than the expression in the well-differentiated RCC specimen (P < .05), respectively. Patients with negative Jade-1 expression and positive β-catenin expression were found to have shorter survival on univariate analysis (P < .05).
CONCLUSION: RCC with a low expression of Jade-1 and high expression of β-catenin is associated with a poor outcome and decreased survival.
Written by:
Lian X, Duan X, Wu X, Li C, Chen S, Wang S, Cai Y, Weng Z. Are you the author?
Department of Urology, Lishui Central Hospital- Fifth Affiliated Hospital of Wenzhou Medical College, Zhejiang, People's Republic of China.
Reference: Urology. 2012 Aug;80(2):485.e7-485.e13.
doi: 10.1016/j.urology.2012.02.024
PubMed Abstract
PMID: 22516360
UroToday.com Investigative Urology Section
