BACKGROUND:The mRNA expression of genes coding enzymes involved in O-GlcNAcylation were analyzed in urine obtained from 176 bladder cancer (BC) patients and 143 healthy persons.
METHODS:MGEA5 and OGT expression was measured by a real-time PCR assay.
RESULTS:OGT expression was not detected in urine of healthy persons but it was found in 51.7% of BC samples. Positive expression of MGEA5 was found in urine of both healthy persons (47.1%) and BC patients (52.3%). Poorly differentiated BC (grade III) showed significantly lower MGEA5 expression than grade I tumors. Contrary, OGT transcript level was significantly higher in grade II and III in comparison to grade I BC. Moreover, there was significant difference in OGT expression between early bladder cancers and invasive or advanced bladder cancers.
CONCLUSIONS: These results suggest that analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics.
Written by:
Rozanski W, Krzeslak A, Forma E, Brys M, Blewniewski M, Wozniak P, Lipinski M. Are you the author?
2nd Department of Urology, Medical University of Lodz, Lodz, Poland.
Reference: Clin Lab. 2012;58(5-6):579-83.
PubMed Abstract
PMID: 22783592
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