BACKGROUND: The transduction efficacy of adenovirus serotype 5 (Ad5) vector in high-grade human bladder cancer cells is generally extremely low due to the non-expression of coxsackie and adenoviral receptor (CAR).
We investigated whether fiber-modified adenovirus vector containing an RGD motif in the HI loop of the adenovirus fiber knob could increase the transduction efficiency of Ad5 into human bladder cancer cells in vitro.
MATERIALS AND METHODS: We examined the expressions of CAR, and of αv, β3 and β5 integrin, and the transduction efficacy of fiber-modified adenovirus vector in four human bladder cancer cell lines (TCC-SUP, 253J, T24 and KK47).
RESULTS: The expression of CAR was lower and those of αv and β3 integrin were higher in four human cancer cell lines compared with the control cell line, KK47. The transduction efficacy of fiber-modified adenovirus vector increased by 20- to 470-fold compared with Ad5.
CONCLUSION: Fiber-modified adenovirus vector may be useful in order to establish new effective gene therapy strategies for the treatment of high-grade human bladder cancer.
Written by:
Hiwasa K, Nagaya H, Terao S, Acharya B, Hamada K, Mizuguchi H, Gotoh A. Are you the author?
Laboratory of Cell and Gene Therapy, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya, Japan.
Reference: Anticancer Res. 2012 Aug;32(8):3137-40.
PubMed Abstract
PMID: 22843884
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