Well-defined tertiary amine-functionalized cationic polylactides (CPLAs) are synthesized by thiol-ene click functionalization of an allyl-functionalized polylactide, and utilized for the delivery of interleukin-8 (IL-8) siRNA via CPLA-IL-8 siRNA nanoplexes.
The CPLAs possess remarkable hydrolytic degradability, and their cytotoxicity is relatively low. The CPLA-IL-8 siRNA nanoplexes can be readily taken up by prostate cancer cells, resulting in significant IL-8 gene silencing. It is found that the degradability and cytotoxicity of CPLAs, as well as the transfection efficiency of the CPLA-IL-8 siRNA nanoplexes, positively correlate with the amine mol% of CPLAs.
Written by:
Chen CK, Law WC, Aalinkeel R, Nair B, Kopwitthaya A, Mahajan SD, Reynolds JL, Zou J, Schwartz SA, Prasad PN, Cheng C. Are you the author?
Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260-4200, USA
Reference: Adv Healthc Mater. 2012 Nov;1(6):751-61
doi: 10.1002/adhm.201200094
PubMed Abstract
PMID: 23184827
