Antiproliferative and Antioxidant Effects of Withania coagulans Extract on Benign Prostatic Hyperplasia in Rats

Benign prostate hyperplasia (BPH) is a common urological disorder in elderly men. Phytotherapy is frequently used to alleviate the symptoms of this condition.

The present study investigated the effect of Withania coagulans extract (WCE), which is known to have antioxidant, anti-inflammatory, antihyperglycemic, and anti-cancer properties, on testosterone-induced BPH in rats.

Forty Wistar rats were divided into five groups (each n = 8): the control group, the untreated BPH group, and three WCE-treated groups (WCE250, 500, and 1000). BPH was induced with 3 mg/kg subcutaneous injections of testosterone propionate for four weeks. WCE was concomitantly administrated by oral gavage. At the end of the induction schedule, the animals were sacrificed and their prostate glands were dissected, weighed, and fixed for histological examination (H&E and proliferating cell nuclear antigen [PCNA] staining). Half of each sample was prepared for measurement of malondialdehyde (MDA) and total antioxidant capacity (TAC) levels in the prostate.

The present study revealed that BPH caused elevation of MDA levels, suppression of TAC levels, and increased PCNA expression in the prostate gland. Interestingly, in a dose-dependent manner, WCE caused decreased MDA levels and increased TAC levels in the prostate gland, compared to the untreated BPH group. Histopathological examinations showed a reduction in PCNA expression in the prostate epithelium of the WCE animals.

W. coagulans inhibits the development of BPH can be useful for the treatment of this condition.

Nephro-urology monthly. 2016 Jan 09*** epublish ***

Maryam Sarbishegi, Mohaddeseh Khani, Saeedeh Salimi, Mohharam Valizadeh, Fereydoon Sargolzaei Aval

Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran., Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran., Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran., Department of Medicinal and Aromatic Plant, High Complex Education of Saravan, Saravan, IR Iran., Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran.