Genetically engineered mouse models of prostate cancer allow for study of disease progression from localized tumor formation through distal metastasis. The anatomy of the mouse prostate differs dramatically from the human prostate, being composed of 4 lobe pairs (anterior, dorsal, lateral, and ventral), making the identification and dissection technically challenging.
While the entire murine prostate and surrounding tissue, including urethra, bladder, seminal vesicles, and associated adipose tissue, can be quickly dissected for en bloc analysis, it is necessary to isolate individual prostate lobes for gene expression studies elucidating the molecular mechanisms of prostate cancer. The procedure as described here includes full color images, allowing the researcher to appreciate the unique prostate morphology and tissue manipulation required to harvest individual prostate lobes. Along with removing all extraneous tissue, the procedure allows for direct comparison of the different prostate lobes by established downstream techniques. Importantly, high quality RNA required for next-generation gene expression analysis can only consistently be obtained from ventral and lateral lobes. Finally, preclinical studies using prostate targeted therapies can be monitored specifically in individual prostate lobes on the histological and gene expression levels. This article is protected by copyright. All rights reserved.
Journal of cellular physiology. 2016 Mar 18 [Epub ahead of print]
Areg Zingiryan, Nicholas H Farina, Kristiaan H Finstad, Janet L Stein, Jane B Lian, Gary S Stein
Department of Biochemistry and University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT., Department of Biochemistry and University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT., Department of Biochemistry and University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT., Department of Biochemistry and University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT., Department of Biochemistry and University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT., Department of Biochemistry and University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT.