Nitrogen permease regulator like-2 (NPRL2) has been proved to be a useful suppressor gene in treating many cancers containing renal cancer based on experiments. Transgenic technology which transfect exogenous NPRL2 gene into cancer cell was used in these experiments. However, this technology has defects, such as gene mutation and lost. Cytoplasmic transduction peptide (CTP) can be used to avoid these defects because it can directly mediate proteins to penetrate cell membrane and specifically locate in cytoplasm. In this article, CTP was used to directly mediate NPRL2 protein into renal cancer cell line 786-O, then cell proliferation was detected by CCK-8 method, cell cycle and apoptosis were detected by flow cytometry, cell invasion and migration ability were detected by Transwell. Bcl-xl, Cyt-c and Caspase-3 were detected by real-time fluorescent quantitative PCR and Western blot for the analysis of related mechanism. The result showed that CTP successfully mediated NPRL2 protein into renal cancer cell and the growth of cell was significantly inhibited. The mechanism may be NPRL2 down-regulate the expression of Bcl-xl which can up-regulate Cyt-c and further activate Caspase-3, and then cascade reaction is caused for cell apoptosis on the classic mitochondrial apoptosis pathway.
Biological chemistry. 2016 May 17 [Epub ahead of print]
Yang Zeng, Xiao-Bo Shi, Zheng-Yong Yuan, Mao Ye, Li Jiang, Zhi-Xiong Chen, Jing Xiong, Wei Tang