HPV is detected in 30 to 50% of invasive penile carcinomas and it is frequently associated with basaloid and warty morphological features. Based on this heterogeneity and different clinical behavior, we hypothesized a variation in their HPV genotypic composition. To test this, we evaluated 177 HPV positive cases: basaloid (114), warty-basaloid (28) and warty (condylomatous) (35) invasive carcinomas. HPV DNA detection and genotyping was performed with PCR using SPF-10/DEIA/LiPA25 system. Nineteen HPV genotypes were detected. High-risk HPVs predominated (96%) and low-risk HPVs were rarely present. Most common genotype was HPV16 followed by HPVs 33 and 35. According to the genotypes identified, 93% of the cases would be covered with current vaccination programs. There was a significant variation in the distribution of HPV16 and non-HPV16 genotypes according to histological subtype. HPV16 was significantly frequent in basaloid (87%) and less frequent in warty carcinomas (61%). This molecular difference, along with their distinctive macro-microscopic and prognostic features, makes basaloid and warty carcinomas unique. The gradual decreasing frequency of HPV16 demonstrated in basaloid, warty-basaloid and warty carcinomas suggest that the basaloid cell, present in those types in decreasing proportions, may be responsible for the differences.
Human pathology. 2023 Jul 08 [Epub ahead of print]
Diego F Sanchez, María José Fernández-Nestosa, Laia Alemany, Sofía Cañete-Portillo, Belén Lloveras, Omar Clavero, Ingrid Rodríguez, Wim Quint, Nubia Muñoz, Silvia de Sanjosé, Francisco Xavier Bosch, Antonio Cubilla, HPV VVAP Study Group
Instituto de Patología e Investigación, Asunción, 1228, Paraguay; Translational Oncogenomics Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester Cancer Research Centre, Manchester, M20 4GJ, UK., Universidad Nacional de Asunción, San Lorenzo, 2111, Paraguay., Institut Català D'Oncologia, Barcelona, 08908, Spain., University of Alabama, Birmingham, AL, 35233, USA., Hospital Del Mar, Barcelona, 08003, Spain., Instituto de Patología e Investigación, Asunción, 1228, Paraguay; Universidad Nacional de Asunción, San Lorenzo, 2111, Paraguay., DDL Diagnostic Laboratory, Rijswijk, 2288, Netherlands., National Cancer Institute of Colombia, Bogotá, 111511-110411001, Colombia., Barcelona Institute for Global Health (ISGlobal), Barcelona, 08036, Spain., Instituto de Patología e Investigación, Asunción, 1228, Paraguay; Universidad Nacional de Asunción, San Lorenzo, 2111, Paraguay. Electronic address: .