Primary culture of human prostate organoids and patient-derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complimentary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease.
Stem cells translational medicine. 2020 Mar 14 [Epub ahead of print]
Anastasia C Hepburn, Emma L Curry, Mohammad Moad, Rebecca E Steele, Omar E Franco, Laura Wilson, Parmveer Singh, Adriana Buskin, Susan E Crawford, Luke Gaughan, Ian G Mills, Simon W Hayward, Craig N Robson, Rakesh Heer
Translational and Clinical Research Institute, Newcastle University Centre for Cancer, Newcastle University, Newcastle upon Tyne, UK., Prostate Cancer UK/Movember Centre of Excellence for Prostate Cancer, Centre for Cancer Research and Cell Biology, Queen's University of Belfast, Belfast, UK., Department of Surgery, NorthShore University HealthSystem, Evanston, IL, USA.