Prostate-Specific Antigen (PSA) based screening of prostate cancer (PCa) needs refinement. The aim of this study was the identification of urinary biomarkers to predict the Prostate Imaging-Reporting and Data System (PI-RADS) score and the presence of PCa prior to prostate biopsy. Urine samples from patients with elevated PSA were collected prior to prostate biopsy (cohort = 99). The re-analysis of mass spectrometry data from 45 samples was performed to identify urinary biomarkers to predict the PI-RADS score and the presence of PCa. The most promising candidates, i.e. SPARC-like protein 1 (SPARCL1), Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1), Alpha-1-microglobulin/bikunin precursor (AMBP), keratin 13 (KRT13), cluster of differentiation 99 (CD99) and hornerin (HRNR), were quantified by ELISA and validated in an independent cohort of 54 samples. Various biomarker combinations showed the ability to predict the PI-RADS score (AUC = 0.79). In combination with the PI-RADS score, the biomarkers improve the detection of prostate carcinoma-free men (AUC = 0.89) and of those with clinically significant PCa (AUC = 0.93). We have uncovered the potential of urinary biomarkers for a test that allows a more stringent prioritization of mpMRI use and improves the decision criteria for prostate biopsy, minimizing patient burden by decreasing the number of unnecessary prostate biopsies.
Scientific reports. 2024 Aug 05*** epublish ***
Blaz Pavlovic, Konstantin Bräutigam, Florence Dartiguenave, Paul Martel, Arnas Rakauskas, Valérie Cesson, Markus Veit, Pascal Oechslin, Alexander Gu, Thomas Hermanns, Karim Saba, Cédric Poyet, Andreas M Hötker, Niels J Rupp, Massimo Valerio, Laurent Derré, Daniel Eberli, Irina Banzola
Department of Urology, University Hospital of Zürich, Frauenklinikstrasse 10, 8091, Zürich, Switzerland., Institute of Tissue Medicine and Pathology, University of Bern, Murtenstrasse 31, 3008, Bern, Switzerland., Department of Urology, Urology Research Unit and Urology Biobank, University Hospital of Lausanne, Rue du Bugnon 46, 1005, Lausanne, Switzerland., Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, 8091, Zurich, Switzerland., Department of Pathology and Molecular Pathology, University Hospital Zürich, 8091, Zürich, Switzerland., Department of Urology, University Hospital of Zürich, Frauenklinikstrasse 10, 8091, Zürich, Switzerland. .