The small-molecule, water-soluble molecular beacon probe 1 is hydrolyzed by the lysate and living cells of human prostate cancer cell lines (LNCaP), resulting in strong green fluorescence.
In contrast, probe 1 does not undergo significant hydrolysis in either the lysate or living cells of human nontumorigenic prostate cells (RWPE-1). These results, corroborated by UV-Vis spectroscopy and fluorescent microscopy, reveal that probe 1 is a sensitive and specific fluorogenic and chromogenic sensor for the detection of human prostate cancer cells among nontumorigenic prostate cells and that carboxylesterase activity is a specific biomarker for human prostate cancer cells.
Written by:
Jiang YL, McGoldrick CA, Yin D, Zhao J, Patel V, Brannon MF, Lightner JW, Krishnan K, Stone WL. Are you the author?
Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN 37614, United States.
Reference: Bioorg Med Chem Lett. 2012 Jun 1;22(11):3632-8.
doi: 10.1016/j.bmcl.2012.04.055
PubMed Abstract
PMID: 22572577
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