Abiraterone acetate is an oral prodrug of abiraterone, a selective inhibitor of CYP17, used for patients with metastatic castration-resistant prostate cancer (mCRPC).
To date, a single liquid chromatographic-tandem mass spectroscopy method has been reported to assay abiraterone concentration in plasma from mCRPC patients. The aim of this study was to develop a simple and sensitive high performance liquid chromatographic (HPLC) method with fluorescence detection for quantification of abiraterone in plasma from mCRPC patients. After protein precipitation with acetonitrile and a liquid-liquid extraction with diethyl ether, abiraterone, and hydroxy-itraconazole (internal standard) were separated on a C8 Xterra® MS column using a mobile phase of acetonitrile and glycine buffer 88.4mM (pH 9.0) (60:40, v/v). Samples were eluted isocratically at a flow rate of 0.9ml/min throughout an 11-min run. Fluorescence wavelengths' excitation and emission were 255 and 373nm, respectively. The calibration was linear in the range 1.75-50ng/ml. Inter- and intraday imprecision were less than 3.5 and 7%, respectively. This method is simple, sensitive, and selective. This analytical method was successfully applied to determine the steady-state plasma exposure to abiraterone in mCRPC patients. This method can be used in routine clinical practice to monitor plasma abiraterone concentrations in mCRPC patients.
Written by:
Belleville T, Noé G, Huillard O, Thomas-Schoemann A, Vidal M, Goldwasser F, Alexandre J, Blanchet B. Are you the author?
Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France; Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Service de Cancérologie médicale, Paris, France; UMR8638 CNRS, Faculté de Pharmacie, Université Paris Descartes, PRES Sorbonne Paris Cité, Paris, France; Institut Cochin, INSERM U 1016, CNRS UMR8104, CARPEM, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
Reference: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 May 1;989:86-90.
doi: 10.1016/j.jchromb.2015.03.001
PubMed Abstract
PMID: 25813901