Prevalence of Germline Mutations in Cancer Susceptibility Genes in Patients With Advanced Renal Cell Carcinoma
To examine the prevalence of germline mutations in both known RCC predisposition genes and other cancer-associated genes and to identify clinical and pathologic factors associated with germline mutations.
In this cohort study conducted from October 1, 2015, to July 31, 2017, 254 of 267 patients with advanced (American Joint Committee on Cancer stage III or IV) RCC who were seen in medical oncology or urology clinics agreed to germline sequencing and disclosure of results under an institutional protocol of matched tumor-germline DNA sequencing.
Mutation prevalence and spectrum in patients with advanced RCC were determined. Clinical characteristics were assessed by mutation status.
Of the 254 patients (median age [range], 56 [13-79] years; 179 [70.5%] male; 211 [83.1%] non-Hispanic white), germline mutations were identified in 41 (16.1%); 14 (5.5%) had mutations in syndromic RCC-associated genes (7 in FH, 3 in BAP1, and 1 each in VHL, MET, SDHA, and SDHB). The most frequent mutations were CHEK2 (n = 9) and FH (n = 7). Of genes not previously associated with RCC risk, CHEK2 was overrepresented in patients compared with the general population, with an odds ratio of RCC of 3.0 (95% CI, 1.3-5.8; P = .003). Patients with non–clear cell RCC were significantly more likely to have an RCC-associated gene mutation (9 [11.7%] of 74 vs 3 [1.7%] of 177; P = .001), and 8 (10.0%) had a mutation in a gene that could guide therapy. Of patients with mutations in RCC-associated genes, 5 (35.7%) failed to meet current clinical guidelines for genetic testing.
Of patients with non–clear cell RCC, more than 20% had a germline mutation, of which half had the potential to direct systemic therapy. Current referral criteria for genetic testing did not identify a substantial portion of patients with mutations, supporting the role of a more inclusive sequencing approach.
Authors: Maria I. Carlo, MD; Semanti Mukherjee, PhD; Diana Mandelker, MD, PhD; Joseph Vijai, PhD; Yelena Kemel, MS, ScM; Liying Zhang, MD, PhD; Andrea Knezevic, MS; Sujata Patil, PhD; Ozge Ceyhan-Birsoy, PhD; Kuo-Cheng Huang, MD; Almedina Redzematovic, MS; Devyn T. Coskey, BS; Carolyn Stewart, BA; Nisha Pradhan, BA; Angela G. Arnold, MS; A. Ari Hakimi, MD; Ying-Bei Chen, MD, PhD; Jonathan A. Coleman, MD; David M. Hyman, MD; Marc Ladanyi, MD; Karen A. Cadoo, MD; Michael F. Walsh, MD; Zsofia K. Stadler, MD; Chung-Han Lee, MD, PhD; Darren R. Feldman, MD; Martin H. Voss, MD; Mark Robson, MD; Robert J. Motzer, MD; Kenneth Offit, MD, MPH
Author Affiliations: Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York (Carlo, Mukherjee, Vijai, Redzematovic, Coskey, Stewart, Pradhan, Arnold, Hyman, Cadoo, Walsh, Stadler, Lee, Feldman, Voss, Robson, Motzer, Offit); Sloan Kettering Institute, New York, New York (Mukherjee, Vijai, Kemel, Offit); Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York (Mandelker, Zhang, Ceyhan-Birsoy, Huang, Chen, Ladanyi); Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, New York (Knezevic, Patil); Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York (Hakimi, Coleman).
JAMA Oncol. 2018;4(9):1228-1235. doi:10.1001/jamaoncol.2018.1986 Published online July 5, 2018.
PubMed http://www.ncbi.nlm.nih.gov/pubmed/29978187