Clear cell renal cell carcinoma (CCRCC) is the most aggressive form of renal cell carcinoma (RCC).
This study was aimed to identify the differentially expressed miRNAs and target genes in CCRCC.
The miRNA and mRNA next-generation sequencing data were downloaded from The Cancer Genome Atlas (TCGA) dataset. Differential expression analysis was performed, followed by correlation analysis of miRNA-mRNA. Functional enrichment and survival analysis was also performed.
Seven hundred and eighty-seven patients with CCRCC from TCGA data portal were included in this study. A total of 52 differentially expressed miRNAs were identified in CCRCC. Then 2361 differentially expressed genes (DEGs) were identified. Prediction analysis and correlation analysis revealed that 89 miRNA-mRNA pairs were not only predicted by algorithms but also had a significant inverse relationship. Several differentially expressed miRNAs such as hsa-mir-501 and their target genes including AK1, SLC25A15 and PCDHGC3 had a significant prognostic value for CCRCC patients.
Alterations of differentially expressed miRNAs and target genes may be involved in the development of CCRCC and can be considered as the prognostic markers for CCRCC.
Cancer biomarkers : section A of Disease markers. 2018 Aug 31 [Epub ahead of print]
Jianmin Liu, Beibei Liu, Yuanyuan Guo, Zhijun Chen, Wei Sun, Wuyue Gao, Hongliang Wu, Yan Wang