Clear cell renal cell carcinoma (CCRCC) is characterized by a highly metastatic potential. The stromal communication between stem cells and cancer cells critically influences metastatic dissemination of cancer cells.
The effect of exosomes isolated from cancer stem cells (CSCs) of CCRCC patients on the progress of epithelial-mesenchymal transition (EMT) and lung metastasis of CCRCC cells were examined.
CSCs exosomes promoted proliferation of CCRCC cells and accelerated the progress of EMT. Bioactive miR-19b-3p transmitted to cancer cells by CSC exosomes induced EMT via repressing the expression of PTEN. CSCs exosomes derived from CCRCC patients with lung metastasis produced the strongest promoting effect on EMT. Notably, CD103+ CSC exosomes were enriched in tumor cells and in lung as well, highlighting the organotropism conferred by CD103. In addition, CD103+ exosomes were increased in blood samples from CCRCC patients with lung metastasis.
CSC exosomes transported miR-19b-3p into CCRCC cells and initiated EMT promoting metastasis. CD103+ acted to guide CSC exosomes to target cancer cells and organs, conferring the higher metastatic capacity of CCRCC to lungs, suggesting CD103+ exosomes as a potential metastatic diagnostic biomarker. ᅟ.
Molecular cancer. 2019 Apr 11*** epublish ***
Lu Wang, Guang Yang, Danfeng Zhao, Jiaqi Wang, Yang Bai, Qiang Peng, Hongzhi Wang, Ruizhe Fang, Guang Chen, Zhichao Wang, Keliang Wang, Guangbin Li, Yinhui Yang, Ziqi Wang, Pengyu Guo, Li Peng, Dayong Hou, Wanhai Xu
Department of Urology (Heilongjiang Key Laboratory of Scientific Research in Urology), the Fourth Hospital of Harbin Medical University, No. 37 Yi-Yuan Street, Nangang District, Harbin, Heilongjiang Province, 150081, People's Republic of China., Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China., Department of Urology (Heilongjiang Key Laboratory of Scientific Research in Urology), the Fourth Hospital of Harbin Medical University, No. 37 Yi-Yuan Street, Nangang District, Harbin, Heilongjiang Province, 150081, People's Republic of China. .