The identification of non-invasive biomarkers for detection of renal cell carcinoma (RCC) in early-stage patients may help improve disease outcome. Certain long noncoding RNAs (lncRNAs) have been reported to be possible biomarkers for diagnosis and prognosis of cancer. Here, we examined the suitability of the lncRNA LINC00887 as a potential biomarker for RCC, as its expression has been shown to be elevated in RCC tissue versus normal tissue in the Gene Expression Profiling Interactive Analysis (GEPIA) database. We found that LINC00887 expression is significantly increased in early-stage RCC tissues and serum of early-stage RCC patients compared with matched normal tissues and serum of healthy subjects, respectively. We also demonstrated that elevated serum LINC00887 is generated from the tumor tissues of RCC patients. Moreover, a receiver operating characteristic (ROC) curve was generated to analyze the diagnostic value of serum LINC00887. An area under the ROC cure differentiating early-stage RCC patients from healthy subjects was 0.8001, with 71.05% sensitivity and 89.87% specificity. Furthermore, we found that LINC00887 promotes RCC cell proliferation in vitro. Together, our findings suggest that a serum LINC00887 signature is associated with RCC cell proliferation and may be a potential biomarker for detection of early-stage RCC.
FEBS open bio. 2020 Jul 12 [Epub ahead of print]
Junjie Xie, Yu Zhong, Chen Rong, Gang Li, Yongwen Luo, Jintao Yang, Zhongwei Sun, Yanzhong Liu, Peipei Liu, Na Wang, Jiaqi An, Chao Li, Yang Song
Department of Urology, Eighth Medical Center of Chinese, PLA General Hospital, Beijin, 100091, China., Department of Urology, 958th Hospital of PLA, Southwest Hospital, Army Medical University, Chongqing, 400020, China., Center of Clinical Laboratory, First medical center of Chinese, PLA General Hospital, Beijin, 100853, China., Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China., Department of Urology, 967th hospital of PLA, Dalian, 116011, China., Outpatient comprehensive treatment area, First medical center of Chinese PLA general hospital, Beijin, 100853, China., Shandong First Medical University, Shandong, 271016, China.