Immune checkpoint inhibitors (ICIs) targeting the programmed cell death protein 1 and its ligand (PD-1/PD-L1) have transformed the treatment paradigm for metastatic renal cell carcinoma (RCC). However, response rates to ICIs as single agents or in combination vary widely and predictive biomarkers are lacking. Possibly related to the heterogeneity and dynamic nature of PD-L1 expression, tissue-based methods have shown limited value. Immuno-positron emission tomography (immunoPET) may enable noninvasive, comprehensive, and real-time PD-L1 detection. Herein, we systematically examined the performance of immunoPET for PD-L1 detection relative to immunohistochemistry (IHC) in an RCC patient-derived tumorgraft (TG) platform.
Eight independent RCC TGs with a wide range of PD-L1 expression (0 - 85%) were evaluated by immunoPET. Uptake of 89Zr-labeled atezolizumab ([89Zr]Zr-DFO-ATZ) was compared to PD-L1 expression in tumors by IHC through double-blind analyses. Clinical outcomes of ICI-treated patients whose TGs were examined were analyzed to evaluate the clinical role of immunoPET in RCC.
ImmunoPET with [89Zr]Zr-DFO-ATZ (day 6/7 post-injection) revealed a statistically significant association with PD-L1 IHC assays (p = 0.0014; correlation ρXY = 0.78). Furthermore, immunoPET can be used to assess the heterogeneous distribution of PD-L1 expression. Finally, studies in the corresponding patients (n = 4) suggest that PD-L1 signal may influence ICI responsiveness.
ImmunoPET with [89Zr]Zr-DFO-ATZ may enable a thorough and dynamic assessment of PD-L1 across sites of disease. The power of immunoPET to predict ICI response in RCC is being explored in an ongoing clinical trial (NCT04006522).
Clinical cancer research : an official journal of the American Association for Cancer Research. 2022 Sep 08 [Epub ahead of print]
Aditi Mulgaonkar, Roy Elias, Layton Woolford, Bing Guan, Kien Nham, Payal Kapur, Alana Christie, Vanina T Tcheuyap, Nirmish Singla, I Alex Bowman, Christina Stevens, Guiyang Hao, James Brugarolas, Xiankai Sun
The University of Texas Southwestern Medical Center, Dallas, TX, United States., Johns Hopkins University School of Medicine, United States., New York Institute of Technology, United States., First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China., The University of Texas Southwestern Medical Center, Dallas, Texas, United States., Banner MD Anderson Cancer Center, Gilbert, AZ, United States., UT Southwestern Medical Center, Dallas, Texas, United States.