Targeted drugs are the main methods of RCC treatment. However, drug resistance is common in RCC patients, in-depth study of the drug-resistant mechanism is essential.
We constructed sunitinib resistant and Twist overexpressed A498 cells, and studied its mechanisms in vitro and in vivo.
In cell research, we found that either sunitinib resistance or Twist overexpression can activate Wnt/β-catenin and EMT signaling pathway, and the sunitinib resistance may work through β-catenin/TWIST/TCF4 trimer. In zebrafish research, we confirmed the similarity of Twist overexpression and sunitinib resistance, and the promoting effect of Twist overexpression on drug resistance.
Sunitinib resistance and Twist overexpression can activate Wnt/β-catenin signaling pathway and EMT to promote the growth and metastasis of RCC cells.
BMC cancer. 2024 Feb 05*** epublish ***
Fangzhen Cai, Jianwei Li, Yanmei Zhang, Sihuai Huang, Wenbin Liu, Weifeng Zhuo, Chengzhi Qiu
Department of Urology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China., Department of Urology, JinJiang Municipal Hospital, Quanzhou, Fujian, China., Department of General Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China. .