Clear cell renal cell carcinoma (ccRCC) is the most prevalent kidney cancer, with a highly aggressive phenotype and poor prognosis. RNA binding proteins (RBPs) play crucial roles in post-transcriptional gene regulation and have been implicated in tumorigenesis. RBPs have the potential to become a new therapeutic target for ccRCC. In this study, we screened and validated that insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) as an RBP, was down-regulated in ccRCC tissues and cell lines. Functionally, we verified that IGF2BP2 significantly suppressed the migration and invasion ability of ccRCC in vitro and in vivo. Mechanistically, RIP-seq and actinomycin D experiments results showed that IGF2BP2 enhanced the expression of Creatine Kinase B (CKB) by binding to CKB mRNA and enhancing its mRNA stability. Thus, IGF2BP2 inhibited ccRCC metastasis through enhancing the expression of CKB. Taken together, these finding suggests that IGF2BP2 is a novel metastasis suppressor of ccRCC and may serve as a potential therapeutic target.
Translational oncology. 2024 Feb 10 [Epub ahead of print]
Junwu Ren, Bo Huang, Wei Li, Yongquan Wang, Xiaojuan Pan, Qiang Ma, Yuying Liu, Xiaolin Wang, Ce Liang, Yuying Zhang, Shimin Wang, Feifei Yang, Haiping Li, Hao Ning, Yan Jiang, Changhong Qin, Ai Ran, Bin Xiao
College of Pharmacy, Chongqing Medical University, Chongqing 400016, PR China., College of Pharmacy, Chongqing Medical University, Chongqing 400016, PR China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563006, PR China., Department of Pharmacy, Chongqing University Cancer Hospital, Chongqing 400030, PR China., Department of Urology, Southwest Hospital, Army Medical University, Chongqing 400038, PR China., College of Pharmacy, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: .