Piwi-interacting RNAs (piRNAs) are small RNAs of 27-30 nucleotides mapping to transposons or clustering in repeat genomic regions. Preliminary studies suggest an important role in cancerogenesis. This study is the first one investigating their prognostic impact in clear cell renal cell cancer (ccRCC) patients.
Three piRNAs (piR-30924, piR-57125, and piR-38756) selected on the basis of initial piRNA microarray analyses were determined using RT-qPCR in non-metastatic (n = 76) and metastatic (n = 30) ccRCC tissue at the time of nephrectomy in comparison to normal renal tissue (n = 77) and tissue from distant ccRCC metastases (n = 13) Primary clinical end points were recurrence-free and overall survival
piR-57125 showed lower expression in metastatic than in non-metastatic tumors, whereas the expression of piR-30924 and piR-38756 increased in metastatic tumors The higher expression of piR-30924 and piR-38756 as well as the lower expression of piR-57125 in metastatic primary tumors were significantly associated with tumor recurrence and overall survival Multivariate Cox regression analyses revealed both piR-30924 and piR-57125 as independent prognostic predictors This impact was even more pronounced in non-metastatic patients
This study demonstrates that the expression levels of these piRNAs in primary non-metastatic and metastatic ccRCC tissue can serve as potential prognostic biomarkers in combination with clinicopathological factors
Journal of experimental & clinical cancer research : CR 2015 Jun 14*** epublish ***
Jonas Busch, Bernhard Ralla, Monika Jung, Zofia Wotschofsky, Elena Trujillo-Arribas, Philipp Schwabe, Ergin Kilic, Annika Fendler, Klaus Jung
Department of Urology, Research Division, University Hospital Charité, Schumannstr 20/12, 10117, Berlin, Germany jonas busch@charite de , Department of Urology, Research Division, University Hospital Charité, Schumannstr 20/12, 10117, Berlin, Germany bernhard ralla@charite de , Department of Urology, Research Division, University Hospital Charité, Schumannstr 20/12, 10117, Berlin, Germany monika jung@charite de , Department of Urology, Research Division, University Hospital Charité, Schumannstr 20/12, 10117, Berlin, Germany zofia wot@gmail com , Laboratorio clinico, Hospital Universitario Virgen Del Rocio, Sevilla, Spain elenatrujilloarribas@gmail com , Center for Musculoskeletal Surgery, University Hospital Charité, Berlin, Germany philipp schwabe@charite de , Institute of Pathology, University Hospital Charité, Berlin, Germany ergin kilic@charite de , Department of Urology, Research Division, University Hospital Charité, Schumannstr 20/12, 10117, Berlin, Germany annika fendler@mdc-berlin de , Department of Urology, Research Division, University Hospital Charité, Schumannstr 20/12, 10117, Berlin, Germany