It has been proposed that 17-β-estradiol (E2) activates estrogen receptor and inhibits renal cell carcinoma (RCC) growth. In the present study we explored the role of E2 and ER in the regulation of RCC growth.
The RCC cell line ACHN was treated by E2 combining with E2 antagonist Fulvestrant or ER knockdown, and cell growth was monitored. Quantitative phosphoproteomics was applied to study the E2 regulated non-genomic phosphorylation changes. Western blotting, immunofluorescence microscopy, and apoptosis assays were used for validation.
E2 induced ER-dependent growth inhibition in RCC cell lines. Quantitative phosphoproteomics revealed that E2 induced both apoptosis and autophagy. Cellular apoptosis was confirmed by altered mitochondrial membrane potential, and ER-dependent autophagosome formation was also found.
Our data revealed the potential dual roles of E2 in regulating RCC growth via autophagy and apoptosis pathways.
Cancer genomics & proteomics. 0000 [Epub]
Kuo-Chiang Chen, Chih-Ming Lin, Chi-Jung Huang, Shao-Kuan Chen, Sheng-Tang Wu, Han-Sun Chiang, Wei-Chi Ku
Graduate Institute of Nutrition and Food Sciences, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C. Graduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C. School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C. Department of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C., Department of Surgery, Cathay General Hospital, Taipei, Taiwan, R.O.C., School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C. Department of Medical Research, Cathay General Hospital, Taipei, Taiwan, R.O.C. Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, R.O.C., Department of Surgery, Sijhih Cathay General Hospital, New Taipei, Taiwan, R.O.C., Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C., Graduate Institute of Basic Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C. Department of Urology, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C. ., School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan, R.O.C. .