We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe.
Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
Written by:
Almeida C, Azevedo NF, Bento JC, Cerca N, Ramos H, Vieira MJ, Keevil CW. Are you the author?
IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Reference: Eur J Clin Microbiol Infect Dis. 2013 Jan 4. Epub ahead of print.
doi: 10.1007/s10096-012-1808-2
PubMed Abstract
PMID: 23288291
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